Biotechnology EOPA Practice Test

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• Which instrument is used to transfer small amounts of liquid by drawing up into a small tube and releasing?
• In the described serial dilution, what is the total volume after the first dilution step?
• Which group is used to establish a baseline by having no variable applied?
• When removing gloves safely, what should you do first?
• Which statement accurately describes a spectrophotometer's function?
• Which document, provided by chemical suppliers, describes the specific properties of a chemical?
• What must pharmacodynamic (PD) and pharmacokinetic (PK) assays demonstrate?
• Which four categories are represented in the NFPA 704 rating system?
• OSHA stands for which U.S. agency?
• Which of the following is a biotechnology career path listed?
• What is Research?
• What are the basic units of the DNA molecule, composed of a sugar, a phosphate, and one of four DNA bases?
• Which set of characteristics best describes living organisms?
• Which statement best describes Direct ELISA?
• Which of the following is one of the MSDS eight sections?
• What is the isoelectric point?
• Column Chromatography is best described as which?
• What is the primary function of lysosomes?
• Which device is used to transfer small amounts of liquid?
• Which organelle contains the DNA and provides instructions for cellular functions?
• Which phase uses healthy volunteers to test safety and dosage?
• Why are dilutions performed?
• Which instrument is an extractor consisting of a pair of pincers used in medical treatment (delivery of babies)?
• Finite cell lines are characterized by which property?
• Which device is used to hold burets on a stand in a lab setup?
• Which biotechnology career field involves computational analysis of biological data?
• Which statement accurately compares typical cell sizes?
• Myosin is best associated with which function?
• Which statement describes stationary phase?
• Which statement best describes hydrogen bonds compared to covalent bonds?
• Which technique uses an iodine vapor chamber for visualization?
• Which item spins rapidly in a rotating field to stir and dissolve?
• Which institutions conduct pure-science research to enrich the scientific knowledge base?
• In recombinant DNA technology, which genetic elements are commonly used as vectors and replicate independently in bacteria?
• Which instrument is used to measure an object's mass to a very high degree of precision?
• What is a cotton or cloth used for removing moisture or discharges from mucous membranes as well as for applying bleaches or liquid disinfectants?
• Which clamp is used to hold burets and is coated with plastic or rubber?
• How should broken glass be handled in the lab?
• Which device is used to separate substances by spinning at high speed?
• Which item is typically used to gently heat samples by immersion in warm water?
• In ion-exchange chromatography, what happens to molecules that do not have opposite charge to the beads?
• What can be called a 'graduated pipette'
• Carbohydrates are best described as which of the following?
• Which term describes a legally exclusive right to an invention for a fixed period?
• Which item is a very fine pipette used for measuring small quantities?
• Which equipment provides protection for the entire face from splashes?
• Which flask is used for heating substances that need to be heated evenly; bulbous bottom allows heat to distribute through the liquid more evenly, mostly used in distillation experiments?
• Which instrument is used to measure and pour exact amounts of a liquid?
• Genetic testing identifies changes in what?
• In a solution, the component that is dissolved is called:
• Type AB blood can donate to which recipients?
• Which item is a very fine pipette for measuring, transferring, or injecting very small quantities of liquid?
• Which organelle contains the DNA and provides instructions for cellular functions?
• Which item is a shallow, circular, transparent dish with a flat lid, used for the culture of microorganisms?
• Which symbol is associated with Biohazardous/Infectious?
• Which statement is true about micropipette accuracy?
• What is recombinant DNA?
• Which statement best describes the goal of affinity chromatography?
• In a PCR setup, what serves as the initial genetic material to be amplified?
• What is a vector?
• Which structure enables bacteria to stick to surfaces and participates in genetic exchange during conjugation?
• In PCR, what term describes a pre-mixed solution containing DNA polymerase, dNTPs, and buffers?
• Which pH range is acidic?
• What is biotechnology?
• Which instrument is used to make solutions of known concentration and provides accurate liquid volume measurement?
• If a measurement is close to the standard value but not reproducible, the result is:
• Structural genes are composed of which parts?
• Which trio of agencies is commonly referenced for product regulatory guidelines?
• Which capability is listed as part of the Diagnostic Research sector?
• Pharmaceuticals in genetically engineered plant crops are associated with which sector?
• Which item is used to protect the hands?
• Which term describes rod-shaped bacteria?
• Which statement describes a concentration assay using colored indicators to estimate protein concentration?
• In Diagnostic Research, which capability is used to store, analyze and compare data?
• Which organelle is responsible for protein production?
• Which scientist established norms and expectations for future vaccine trials while developing the whooping cough vaccine?
• Which term describes spiral-shaped bacteria?
• Continuous cell lines are characterized by which property?
• Which glassware is typically conical in shape and used for mixing liquids to minimize splashes?
• Which instrument is used to measure temperature?
• In biotechnology, which category is most likely to bring products to market and generate profits?
• Which statement about incubators is correct beyond the basic purpose?
• Which action is explicitly listed as part of aseptic technique?
• What is a characteristic of Indirect ELISA?
• What is the shape of DNA?
• Which symbol is identified as Harmful or Fatal?
• In the NFPA 704 rating system, what does the blue section indicate?
• Conjugation in bacteria is best described as:
• Which structure forms the outer boundary that regulates movement of materials into and out of the cell?
• Which statement best describes Gram-negative bacteria?
• Which option defines buffers?
• Which item is designed to hold test tubes upright in a laboratory?
• What is an agar plate used for?
• Which statement best describes antibody structure and specificity?
• Which item provides eye protection from impact of foreign objects?
• What does an activity assay demonstrate?
• Diafiltration is defined as which?
• In affinity chromatography, which statement best describes what happens to the protein of interest?
• The Rh factor refers to the presence or absence of which antigen?
• Which item spins very quickly within a solution to stir and accelerate the dissolving process?
• Which piece of PPE provides full-face protection in the lab?
• What does molecular weight measure?
• Which ELISA format is characterized by the antigen being pinched between two antibodies?
• Collagen provides structural support for which of the following?
• Which device picks up and holds test tubes; the test tubes may be hot
• Which level of protein structure reflects interactions among multiple polypeptide chains?
• Which instrument is used to make incisions and excisions?
• Which statement is true about Rh compatibility in transfusions?
• Which scientist established bacteriology as a science and worked with Frederich Loeffler to develop a method to identify a disease-causing agent?
• Which item is a cylinder-shaped, calibrated container used in centrifugation to hold samples?
• Which process enables genetic material transfer between bacteria, increasing genetic diversity?
• What are restriction enzymes?
• What is pKa?
• Confluence in cell culture refers to what?
• Which statement best describes how prokaryotes are used in biotechnology?
• What does a health rating of 4 indicate in the NFPA 704 classification?
• Which item is described as a narrow, usually calibrated tube used to suction small amounts of liquid for transfer or measurement?
• Which feature distinguishes Gram-positive bacteria from Gram-negative bacteria?
• Which components provide the building blocks for DNA synthesis in PCR?
• Which equipment spins liquid samples at high speed to separate components?
• In size-exclusion chromatography, what feature of the resin is responsible for separating molecules by size?
• A __ is a form of intellectual property that gives its owner the legal right to exclude others from making, using, or selling an invention for a limited period of years.
• What is molarity?
• Which term describes the outer boundary that regulates movement of materials into and out of the cell?
• Which process is not used by prokaryotes to transfer DNA?
• Adherent cultures are characterized by what behavior?
• Which description best characterizes antiparallel orientation of the DNA strands?
• In which decade did the first GMO foods become available for human consumption?
• What is Development?
• Which MSDS section provides hazard ingredients/identity?
• Pipette Pump, volume of 10 mL is which color?
• Which instrument is used to transfer liquids into containers with small openings and has a wide mouth and narrow spout?
• What does ELISA primarily measure?
• When adding a solid to a liquid, what is the recommended approach?
• Which statement about Gram staining is true?
• Which component helps maintain pH and ionic strength during PCR?
• Which term describes the set of steps a product must go through to reach the market?
• In the scientific method, which step follows formulating the hypothesis?
• Which statement correctly describes the formation of ionic bonds?
• Which symbol is used for corrosive substances?
• Which organelle is a protein network in the cytoplasm that provides structural support?
• Which stage in PCR is used to separate DNA strands by heating?
• Which statement best describes lipids?
• In the NFPA rating system, what does a 0 rating in the red section signify?
• Gene therapy is defined as
• GLP is the quality system used to collect safety data on a product being developed for non-clinical health and environmental safety studies.
• Which description best defines the nucleus?
• Which symbol represents Corrosive hazards?
• Which instrument is a two-piece embalming instrument consisting of a handle and a blade used to make incisions?
• Pipette Filler-Dispenser is best described as which of the following?
• GLP stands for?
• Which statement about ELISA formats is true?
• Which microscope slide is curved inward or hollowed?
• Which statement about micropipettes is accurate?
• What does the red section of the NFPA diamond indicate?
• Which MSDS section would you consult for precautions for safe handling and use?
• What is the purpose of stability assays?
• Clinical trial phase that investigates whether the drug works and the safety on people who are suffering from the disease
• Which option describes chromatography?
• Nucleic acids are present in living cells and include DNA and RNA.
• pH neutral corresponds to which value?
• Which item is used to retrieve an inoculum from a culture of microorganisms?
• Which statement best describes death phase?
• What are the molecules that carry genetic information in living organisms?
• Which agency is responsible for checking the safety of food and drugs?
• Which entities conduct pure or applied science to develop products for the treatment of major diseases?
• Thin-Layer Chromatography uses which of the following?
• Hydrophilic describes substances that tend to do what with water?
• Which tool is used to draw liquids up into a pipet tube and is squeezed to perform actions?
• Which symbol indicates Explosion Hazard?
• What is a typical initial step when using a thermal cycler?
• Which simple tool is used to retrieve an inoculum from a culture of microorganisms?
• Which symbol corresponds to Corrosive?
• What is Formula Weight?
• In chromatography, which option best describes Column Chromatography where components separate as they pass through a column?
• Which statement correctly describes carbohydrates?
• Which flask is used to heat and store liquids with a bottom wider than the top to heat quicker because of greater surface area?
• Which entities produce and sell commercial products?
• Which step in manufacturing involves demonstrating 'proof of concept' and being assessed to determine if the product could be developed safely and effectively?
• Which tool is used to pick up and hold test tubes, including hot ones?
• Organisms with altered genomes are known as what?
• Which assays are designed to demonstrate activity in animals and relate to the amount and duration of effect?
• What is an open tube used to hold liquids?
• What is a cell strain?
• Three rounds of clinical trials are designed to test progressively larger numbers of patients.
• Which term describes a product being developed and undergoing clinical trials?
• Which disposable item is used to transfer small volumes of liquid?
• What distinguishes molecular weight from formula weight?
• Which term describes the closeness of repeated measurements to each other?
• Which statement reflects proper use of a laboratory balance?
• Which of the following is listed as a proper laboratory behavior?
• What is a plasmid?
• Which statement correctly describes Indirect ELISA?
• Which biotech sector includes gene therapy and pharmacogenomics?
• Which technique uses a column packed with an adsorbent such as silica gel or alumina to separate components?
• In ion-exchange chromatography, which molecules will attach to the beads?
• What is the primary purpose of an incubator in a biotechnology laboratory?
• Which disposable item is used to transfer very small volumes of liquid, often for single use?
• In which biotechnology sector are DNA sequencing, genomics, proteomics, and bioinformatics activity typically grouped?
• Which term describes the basic units of the DNA molecule that include a sugar, a phosphate, and a nitrogenous base?
• Accuracy is defined as:
• Which molecule contains the sugar ribose and is typically single-stranded?
• Secondary structure in proteins is formed by hydrogen bonds between amino acids in close proximity. Which structures are formed?
• Which statement about prokaryotic cells is true?
• Which statement describes Paper chromatography?
• Which safety practice is appropriate when using a hot water bath?
• Which of the following describes measurements not accurate but precise?
• Which enzyme is commonly used in PCR to synthesize DNA?
• Industrial and Environmental biotechnology includes activities such as fermentation of foods and beverages and production of enzymes for industry.
• Why are dilutions performed?
• Which statement indicates sterilization status when using autoclave tape?
• The autoclave is a device for heating substances above their boiling point.
• Which statement best describes how an agar plate is used in microbiology?
• Which growth phase is characterized by rapid replication and doubling times of about 20 minutes?
• What is the Human Genome Project?
• What is the shape of DNA?
• What do concentration assays estimate?
• Small accessory chromosomes found in bacteria and useful in recombinant DNA are called what?
• Phase III trials are conducted in which context?
• In a Sandwich ELISA, how is the antigen captured and detected?
• In electrophoresis, which of the following is correct about electrode placement?
• What is the nucleolus?
• Covalent bonds form when two atoms have which characteristic?
• Who uses laboratory techniques to analyze evidence?
• Which description correctly defines binary fission?
• Phase I clinical trials are typically conducted with which group?
• What term describes spherical bacteria?
• Which symbol is identified as Oxidizing?
• Which statement about exponential growth is true?
• Which technique is used to separate DNA fragments to create a DNA fingerprint?
• Which disposal practice is explicitly stated as appropriate for bio-hazardous materials?
• Keratin is a structural protein found in which tissues?
• Which federal agency funds and conducts biomedical research?
• Which symbol corresponds to Compressed Gas?
• Which term describes the procedure by which a subject voluntarily agrees to participate in a clinical trial after understanding all information?
• Which of the following is a carbohydrate?
• What is pH?
• Which device uses centrifugal force to separate particles from a suspension?
• The substance in which the solute dissolves is called:
• Ethics is defined as which of the following?
• What term refers to accessory rings of DNA that can be used as vectors to carry foreign genes into bacteria during genetic engineering?
• Lipids are best described as what?
• What does NFPA stand for?
• Who developed the polio vaccine using a double-blind randomized experiment?
• Which symbol is labeled Harmful or Fatal?
• What information should be included on a label to be correct?
• Which structure provides a rigid structural support around the cell membrane?
• Weighing dishes/boats are used for what purpose?
• Which term describes a statement proposed to be tested in an experiment?
• Hydrogen bonds form when hydrogen bonds with which elements?
• Chloroplasts are disk-shaped organelles found in which cells?
• What is the role of the ribosome in translation?
• What decade did the first GMO food become available for human consumption?
• Clinical trial phase that monitors the effectiveness and side effects of the drug
• Which item is a cylinder-shaped, calibrated glass container that fits into centrifuge slots for the analysis and separation of materials?
• Used to test hypothesis
• Which feature is typical of eukaryotic cells?
• What technique allows amplification of genes from a DNA sample?
• When preparing solutions, which practice is recommended for combining acid and base?
• Which practice is recommended when using a spectrophotometer to ensure proper operation?
• Which technique is used to observe the different pigments in a material?
• Which group lacks what is being tested and is used to provide the expected results?
• Which instrument is specifically used to prepare solutions of known concentration by delivering a precise final volume?
• Which device is designed to limit exposure to hazardous fumes, vapors, or dusts?
• Which item is considered specialized equipment needed for PCR?
• What orientation is correct when inserting cuvettes into a spectrophotometer?
• What are the five nucleotides?
• Pipette Pump, volume of 2 mL is which color?
• A true statement about lipids is that they store energy.
• Which agency monitors the safety and quality of poultry, eggs, and meat products?
• GLP requirements include which of the following elements?
• Which instrument is used to clean the interior of test tubes with bristles?
• Which instrument must not be heated?
• Which device is used to suction liquids into or expel liquids out of pipettes?
• Cohen, Boyer, and Berg demonstrated the first recombinant DNA in which organism?
• How is the dilution factor defined?
• Which instrument is used to hold substances while weighing?
• Which statement is true about protein structure levels?
• Which flask has a bulbous bottom and is ideal for even heating, commonly used in distillation?
• Which step in manufacturing involves teams discussing their work, formulating new ideas, and creating a comprehensive product development plan?
• Which statement correctly identifies carbohydrates?
• Which practice is required before starting a centrifuge run?
• Which nutrient category is used by the body to build and maintain its cells and tissues?
• Which symbol indicates Flammable material?
• Which agency is responsible for safe and healthy working conditions for employees?
• DNA and RNA are examples of what?
• Which MSDS section lists physical chemical characteristics?
• Which term describes the gel-like interior outside the nucleus?
• Which expression is used for calculating dilutions?
• What is DNA ligase?
• Which term describes basic units of DNA that include a sugar, a phosphate, and a nitrogenous base?
• Which governing agencies oversee and establish regulatory guidelines for products?
• Nucleic acids are polymers made of what building blocks?
• What is the opposite arrangement of the sugar-phosphate backbones in a DNA double helix?
• In cloning experiments, what is the primary purpose of a vector?
• Precision is defined as:
• Which action is a proper use of a microscope when observing a slide?
• During RNA processing, what happens to introns?
• Which biotechnology sector covers transgenic plants and animals engineered to express a specific gene?
• Proteins are essential to living organisms and contribute to structure.
• Which organelle is the powerhouse of the cell?
• Which MSDS section covers health hazards?
• Which device is an automated pipetting system that can perform programmed transfers of liquids between preselected groups of containers?
• In transcription, a segment of DNA is copied into which molecule?
• Toxicology assays determine which quantities are toxic and help determine dosage. Which option best captures this idea?
• Which level of protein structure describes the arrangement of multiple polypeptide chains forming a functional protein complex?
• SOP stands for?
• SOP is a step-by-step outline for how to perform a particular lab procedure or method.
• Translation initiation begins when the ribosome binds to mRNA and encounters which start codon?
• Which item is commonly used to transfer a precise liquid volume and comes in common sizes such as 10 mL or 25 mL?
• In size-exclusion (gel-filtration) chromatography, which molecules pass the column the fastest?
• BSL-2 is suitable for which institutions?
• Which pH range is considered basic?
• A statement or possible explanation that attempts to answer a scientific question?
• After processing, where does the mRNA molecule go and what does it do?
• Which device is a large beaker of water heated on a hot plate used to heat test tubes containing flammable liquids?
• BSL-1 is typically used in which setting?
• Which membrane-bound organelle is responsible for breaking down cellular waste?
• Which statement best describes alpha helices?
• Which forces stabilize alpha helices and beta pleated sheets?
• What is the primary function of an autoclave?
• Which statement about Type O blood is true?
• RNA polymerase moves along the DNA in which direction during transcription?
• Which statement correctly associates BSL-3 and BSL-4 with the types of facilities described?
• Which description best matches the Golgi apparatus?
• In biotechnology contexts, which type of organization is typically associated with the CDC and NIH?
• Which bacterial structure is described as a sticky layer that helps evade the immune system and adhere to substances?
• What is the main function of chloroplasts in plant cells?
• Which item is described as a reservoir with ten sections, designed to hold tubes and maintain volume calculations?
• Which of the following describes a simple assay?
• Which group is used when the tested variable is applied to compare outcomes against expectations?
• Biotechnology began when humans began which activities?
• Which item is a tiny disposable tip used with automated pipettes?
• Which method is a common way prokaryotes transfer DNA?
• What term describes rod-shaped bacteria?
• What do potency assays evaluate?
• Which instrument is described as a slender, calibrated tube used to measure the volume or transfer a particular quantity of liquid from one container to another?
• Which symbol is associated with Harmful or Fatal hazard?
• Which arrangement describes bacteria that grow in long chains?
• Which term describes spiral-shaped bacteria?
• Which method of DNA replication produces molecules with one parental strand and one new strand?
• Which clinical trial phase uses healthy volunteers to test safety and dosage?
• Which term refers to the long strands of DNA intertwined with protein molecules?
• Which organelle is commonly referred to as the powerhouse of the cell?
• Phenolphthalein and bromothymol blue are used for what?
• What term describes a product being developed and undergoing clinical trials?
• Which symbol is labeled Explosion Hazard?
• During stationary phase, which statement is true regarding resuming growth when transferred to fresh nutrients?
• What does PPE stand for in laboratory safety?
• What term refers to a disposable tip for automated pipettes?
• Which lab tool is a plastic qualitative pipet used to dispense chemicals and is often used in place of a dropper?
• What term refers to the group of laws that regulates the ownership and use of creative works?
• Which regulation protects individually identifiable information about a patient's health?
• Which statement best describes proteins?
• What is the major function of the endoplasmic reticulum?
• Phase II of clinical drug development involves testing the drug in which population?
• Which sequence best describes the Scientific Method?
• Which statement is based on experimental data, error analysis, and suggestions for applications?
• Which item is described as a rack for holding up lab equipment when not in use, or for observation?
• Which organelle contains genetic material and is essential for cell function?
• Which combination describes measurements that are not accurate but are precise?
• What are Clinical trials?
• Prosthetics, artificial or engineered organs and tissues; gene therapy; pharmacogenomics are characteristics of which biotechnology sector?
• Which term describes spherical bacteria?
• Which term describes the 3D shape of a polypeptide chain when it folds?
• Which tool is used to heat substances on a lab bench?
• In the described serial dilution, what is the dilution factor per step?
• Which statement about conjugation is true?
• Which statement about pH meters is true?
• Hydrophobic describes substances that tend to do what with water?
• Which paper shows a specific color change as the pH of a solution changes?
• What is the main function of ribosomes?
• Which instrument is used to draw liquids up into a pipet and is circular and squeezed to perform actions?
• Which item is a rack for holding up lab equipment when not in use, or for observation?
• Which lab instrument is adjustable for any volume and uses a plunger to control delivery of fluid?
• Which parts of the gene remain on the mature mRNA after intron removal?
• Which term means respect for the inherent worth and uniqueness of individuals and populations?
• Which organisms are included in microbial culture (prokaryotic)?
• Which statement best describes Gram-positive bacteria?
• During lag phase, which describes bacterial behavior?
• Which item is an open tube used to hold liquids?
• Which genetic element serves as small, circular DNA separate from the bacterial chromosome and is often used as a cloning vector?
• Cloning refers to which process?
• Which instrument is commonly called a graduated pipette?
• Exponential phase is characterized by:
• Why are plasmids often used in laboratory gene manipulation?
• Who developed the smallpox vaccine by testing cowpox on the gardener's son?
• In a solution, formation occurs when a solute is dissolved in a solvent. Which option represents the outcome?
• What does the primary structure of a protein refer to?
• Which term describes a single-stranded nucleic acid that contains the sugar ribose?
• Which term best describes the gel-like interior of the cell outside the nucleus?
• Solute is defined as:
• Which instrument is best described as a narrow, tall container used for accurate measurement of liquid volume?
• Which agency coordinates programs aimed at reducing pollution and protecting the environment?
• What is the purpose of an agar slant tube in microbiology?
• Which bacterial feature is a gelatinous coating that protects against phagocytosis and assists adhesion to surfaces?
• Which combination best describes measurements that are both correct and consistently close to each other?
• Which container is used to store liquid or gas for an experiment?
• Solvent is defined as:
• What is used to agitate liquids or mixtures?
• Which term refers to long strands of DNA intertwined with protein molecules?
• Which glass container has a cylindrical shape and a pouring lip, used especially in laboratories?
• What is the specific function of DNA ligase in genetic engineering?
• Which structure forms a thick internal wall around DNA to allow dormancy during harsh environmental conditions?
• Which enzyme binds to the promoter region to initiate transcription?
• What is the primary function of mitochondria?
• Which group contains the tested variable and will give the predicted results?
• If repeated measurements are close to each other but far from the standard, the result is:
• Phase I clinical trials are typically conducted with which group?
• Which of the following are examples of nucleic acids?
• Which tool is used to clean out test tubes?
• Which technique uses a coated glass or plastic plate for separation visualization?
• Which statement defines a GMO?
• MSDS stands for which document?
• Which arrangement describes bacteria that occur in pairs?
• What is the primary purpose of the NFPA 704 rating system?